An Elevation of Brain-type Creatine Kinase Isoenzyme in Dogs with Brain Disease
نویسندگان
چکیده
منابع مشابه
Measurement of brain-specific creatine kinase isoenzyme activity in serum.
We have modified the columti-chromatographic technique of Mercer (1) to produce a sensitive and rapid separation of serum CK2 and CK3, even at normal total serum CK activity (3).12 However, CKj was not separated from CK2. Here, we report a modification of our method such that all three CK isoenzymes are separated by stepwise elution from a mini-column of diethylaminoethyl-cellulose. CK1 has onl...
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Creatine kinase (CK) BB, a member of the CK gene family, is a predominantly cytosolic CK isoform in the brain and plays a key role in regulation of the ATP level in neural cells. CK BB levels are reduced in brain regions affected by neurodegeneration in Alzheimer's disease (AD), Pick's disease, and Lewy body dementia, and this reduction is not a result of decreased mRNA levels. This study demon...
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Creatine kinase (EC 2.7.3.2) of rabbit brain (BB) was purified to hemogeneity as judged by sedimentation velocity analysis in the ultracentrifuge. Nevertheless, the purified enzyme contained as many as seven extraneous protein species when examined by disc electrophoresis in polyacrylamide gel. The purified preparation also showed antigenic heterogeneity when it reacted in agar gel double immun...
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ژورنال
عنوان ژورنال: Journal of the Japan Veterinary Medical Association
سال: 1995
ISSN: 0446-6454,2186-0211
DOI: 10.12935/jvma1951.48.491